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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX23257 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23257, RRID:AB_11168829
- Product name
- p53 (phospho Ser392) antibody
- Antibody type
- Polyclonal
- Reactivity
- Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k using p53 (phospho Ser392) antibody (GTX23257) p53 phosphorylated at Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: £f-Ptase). The blot is identical to the control except that it was incubated in £f-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with £f-Ptase.
