Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 701270 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Acetyl-p53 (Lys382) Recombinant Rabbit Monoclonal Antibody (10 H13L14)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 10 H13L14
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Impact of structurally diverse BET inhibitors on SIRT1.
Tenhunen J, Kokkola T, Huovinen M, Rahnasto-Rilla M, Lahtela-Kakkonen M
Gene 2020 May 30;741:144558
Gene 2020 May 30;741:144558
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p53 in whole cell extracts from HeLa cells treated with doxorubicin (0 using a p53 recombinant rabbit monoclonal antibody (Product # 701270) at a dilution of 2 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Samples were detected using chemiluminescence (ECL). Results show a band at ~53kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Acetyl-p53 Lys382 in HeLa cells treated with 0.2uM Doxorubicin and 5mM Sodium Butyrate for 24 hrs using an Acetyl-p53 Lys382 recombinant rabbit monoclonal antibody (Product # 701270) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of acylated p53 and Image E is a composite image of cells showing inhibition of antibody binding after competition with acylated peptide.