701270

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

701270

Provider

Invitrogen Antibodies

Product name

Acetyl-p53 (Lys382) Recombinant Rabbit Monoclonal Antibody (10 H13L14)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Antibody clone number

10 H13L14

Concentration

0.5 mg/mL

References

Submitted references

Impact of structurally diverse BET inhibitors on SIRT1.

Tenhunen J, Kokkola T, Huovinen M, Rahnasto-Rilla M, Lahtela-Kakkonen M
Gene 2020 May 30;741:144558

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p53 in whole cell extracts from HeLa cells treated with doxorubicin (0 using a p53 recombinant rabbit monoclonal antibody (Product # 701270) at a dilution of 2 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Samples were detected using chemiluminescence (ECL). Results show a band at ~53kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Acetyl-p53 Lys382 in HeLa cells treated with 0.2uM Doxorubicin and 5mM Sodium Butyrate for 24 hrs using an Acetyl-p53 Lys382 recombinant rabbit monoclonal antibody (Product # 701270) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of acylated p53 and Image E is a composite image of cells showing inhibition of antibody binding after competition with acylated peptide.