- 13-4000 - Invitrogen Antibodies TP53 antibody

Submitted references CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.

Li S, Liu N, Piao J, Meng F, Li Y
OncoTargets and therapy 2020;13:12383-12395

Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow.

Johnson RW, Finger EC, Olcina MM, Vilalta M, Aguilera T, Miao Y, Merkel AR, Johnson JR, Sterling JA, Wu JY, Giaccia AJ
Nature cell biology 2016 Oct;18(10):1078-1089

Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53.

Martinez-Zapien D, Ruiz FX, Poirson J, Mitschler A, Ramirez J, Forster A, Cousido-Siah A, Masson M, Vande Pol S, Podjarny A, Travé G, Zanier K
Nature 2016 Jan 28;529(7587):541-5

Negative Regulation of p21Waf1/Cip1 by Human INO80 Chromatin Remodeling Complex Is Implicated in Cell Cycle Phase G2/M Arrest and Abnormal Chromosome Stability.

Cao L, Ding J, Dong L, Zhao J, Su J, Wang L, Sui Y, Zhao T, Wang F, Jin J, Cai Y
PloS one 2015;10(9):e0137411

Chimeras of p14ARF and p16: functional hybrids with the ability to arrest growth.

Williams RT, Barnhill LM, Kuo HH, Lin WD, Batova A, Yu AL, Diccianni MB
PloS one 2014;9(2):e88219

Ceramide modulates pre-mRNA splicing to restore the expression of wild-type tumor suppressor p53 in deletion-mutant cancer cells.

Patwardhan GA, Hosain SB, Liu DX, Khiste SK, Zhao Y, Bielawski J, Jazwinski SM, Liu YY
Biochimica et biophysica acta 2014 Nov;1841(11):1571-80

Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation.

Frazzi R, Valli R, Tamagnini I, Casali B, Latruffe N, Merli F
International journal of cancer 2013 Mar 1;132(5):1013-21

Increase in clusterin forms part of the stress response in Hodgkin's lymphoma.

Frazzi R, Casali B, Iori M, Nicoli D, Mammi C, Merli F
International journal of oncology 2011 Mar;38(3):677-84

Cloning and characterization of the novel chimeric gene p53/FXR2 in the acute megakaryoblastic leukemia cell line CMK11-5.

Kanezaki R, Toki T, Xu G, Narayanan R, Ito E
The Tohoku journal of experimental medicine 2006 Jul;209(3):169-80

Cloning and characterization of the novel chimeric gene p53/FXR2 in the acute megakaryoblastic leukemia cell line CMK11-5.

Kanezaki R, Toki T, Xu G, Narayanan R, Ito E
The Tohoku journal of experimental medicine 2006 Jul;209(3):169-80

Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice.

Elso CM, Lu X, Culiat CT, Rutledge JC, Cacheiro NL, Generoso WM, Stubbs LJ
Human molecular genetics 2004 Nov 15;13(22):2813-21

p73alpha regulation by Chk1 in response to DNA damage.

Gonzalez S, Prives C, Cordon-Cardo C
Molecular and cellular biology 2003 Nov;23(22):8161-71

Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue.

Barshack I, Goldberg I, Chowers Y, Weiss B, Horowitz A, Kopolovic J
Journal of clinical pathology 2001 Sep;54(9):684-8

Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue.

Barshack I, Goldberg I, Chowers Y, Weiss B, Horowitz A, Kopolovic J
Journal of clinical pathology 2001 Sep;54(9):684-8

Expression of alternatively-spliced MDM2 transcripts in giant cell tumours of bone.

Evdokiou A, Atkins GJ, Bouralexis S, Hay S, Raggatt LJ, Cowled PA, Graves SE, Clayer M, Findlay DM
International journal of oncology 2001 Sep;19(3):625-32

Expression of alternatively-spliced MDM2 transcripts in giant cell tumours of bone.

Evdokiou A, Atkins GJ, Bouralexis S, Hay S, Raggatt LJ, Cowled PA, Graves SE, Clayer M, Findlay DM
International journal of oncology 2001 Sep;19(3):625-32

p53 represses androgen-induced transactivation of prostate-specific antigen by disrupting hAR amino- to carboxyl-terminal interaction.

Shenk JL, Fisher CJ, Chen SY, Zhou XF, Tillman K, Shemshedini L
The Journal of biological chemistry 2001 Oct 19;276(42):38472-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of p53 was performed by loading 20 µg of HCT 116 (lane1), MDA-MB-231 (lane2), Raji (lane3), Jurkat (lane4) and Jurkat treated overnight with 20 µM of Staurosporine (lane5) cell lysate using NuPAGE® Novex® 4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. p53 was detected at 53 and 73 kDa using p53 Mouse Monoclonal Antibody (Product # 13-4000) at 1:1000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Additional band might correspond to p73 which belongs to the p53 superfamily and exhibits similarity in structure and function.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of p53 showing staining in the nucleus of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p53 monoclonal antibody (Product # 13-4000) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of p53 was done on serum starved HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with p53 Mouse Monoclonal Antibody (134000, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor¨ 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 4 INO80 complex binds two p53 binding sites on the p21 promoter region in 293T cells. ( A ) Six primer sets in the p21 locus used for amplifying ChIP'd DNA. ( B ) q PCR products from each primer set were subjected to 2.5% agarose gel and visualized by ethidium bromide. ( C ) Co-occupying of the INO80 complex and p53 at the -2.2kb and -1.0kb upstream of the p21 transcriptional start site. ChIP assays were performed using INO80, YY1, and p53 antibodies in 293T cells. ChIP'd DNA was analyzed by qPCR. Bar graphs show the ratios of ChIP'd DNA signals to IgG (all signals normalized to input). Error bars represent the standard error of the mean of 3 independent experiments. ( D ) Validation of the recruitment of the INO80 complex at the p21 promoter region. pBS-Vector and pBS-shINO80 transfected 293T cells (48 hours) were used in ChIP assays. ChIP'd DNA with INO80 and YY1 antibodies was analyzed by qPCR. Relative-fold enrichment vs IgG at the -2.2kb and -1.0kb upstream of the p21 transcriptional start site was displayed as bar graphs (all signals normalized to input). Error bars represent the standard error of the mean of 3 independent experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 6 Negative regulation of p21 by INO80 complex is in a p53-dependent manner. ( A ) Significant up-regulation of p21 by shINO80 in p53FL over-expressed p53-/- HCT116 cells. p53-/- HCT116 cells were co-transfected with 0.5 mug p53FL pcDNA (pcDNA3.1 as control) and 1.0 or 2.0 mug pBS-shINO80 plasmids. 48 hours transfection, proteins were analyzed by WB with indicated antibodies. ( B ) Binding of INO80 to p21 gene in p53+/+ HCT116 cells. ChIP assays were performed using INO80 antibody in p53+/+ or p53-/- HCT116 cells. ChIP'd DNA was analyzed by qPCR. Bar graph shows the ratios of ChIP'd DNA signals to IgG (all signals normalized to input). Error bars represent the standard error of the mean of 2 independent experiments. ( C ) Re-ChIP Experimental procedure. ( D ) Binding of INO80 and p53 to the same promoter region of p21 gene in 293T cells. A sequential ChIP according to the experimental procedure shown in C was carried out using INO80 and p53 antibodies in 293T cells. ChIP'd DNA was analyzed by qPCR (all ChIPs were normalized to input). Bar graphs show the relative fold enrichment of the INO80 and p53 vs IgG. Error bars represent the standard error of the mean of 2 independent experiments.

13-4000

Antibody data