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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Other assay [4]
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- Product number
- 13-4100 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p53 Monoclonal Antibody (PAb 240)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- 13-4100 was successfully used to detect p53 in WB, IHC and IP applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PAb 240
- Vial size
- 100 µg
- Concentration
- 0.50 mg/mL
- Storage
- -20°C
Submitted references Yin Yang 1 is critical for mid-hindbrain neuroepithelium development and involved in cerebellar agenesis.
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Pooled screening for antiproliferative inhibitors of protein-protein interactions.
Coordinated control of senescence by lncRNA and a novel T-box3 co-repressor complex.
Mitochondrial dysfunction in Pten haplo-insufficient mice with social deficits and repetitive behavior: interplay between Pten and p53.
UV-induced ablation of the epidermal basal layer including p53-mutant clones resets UV carcinogenesis showing squamous cell carcinomas to originate from interfollicular epidermis.
The effect of the aqueous extract of the roots of Asparagus racemosus on hepatocarcinogenesis initiated by diethylnitrosamine.
Conformationally altered p53: a novel Alzheimer's disease marker?
Uveal spindle cell tumor of blue-eyed dogs: an immunohistochemical study.
A novel loss-of-function mutation in TP53 in an endometrial cancer cell line and uterine papillary serous carcinoma model.
Delayed activation of Bax by DNA damage in embryonic stem cells with knock-in mutations of the Abl nuclear localization signals.
A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers.
The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model.
The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model.
Ionizing radiation-induced apoptosis via separate Pms2- and p53-dependent pathways.
Dong X, Kwan KM
Molecular brain 2020 Jul 23;13(1):104
Molecular brain 2020 Jul 23;13(1):104
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Waddingham MT, Sonobe T, Tsuchimochi H, Edgley AJ, Sukumaran V, Chen YC, Hansra SS, Schwenke DO, Umetani K, Aoyama K, Yagi N, Kelly DJ, Gaderi S, Herwig M, Kolijn D, Mügge A, Paulus WJ, Ogo T, Shirai M, Hamdani N, Pearson JT
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
Pooled screening for antiproliferative inhibitors of protein-protein interactions.
Nim S, Jeon J, Corbi-Verge C, Seo MH, Ivarsson Y, Moffat J, Tarasova N, Kim PM
Nature chemical biology 2016 Apr;12(4):275-81
Nature chemical biology 2016 Apr;12(4):275-81
Coordinated control of senescence by lncRNA and a novel T-box3 co-repressor complex.
Kumar P P, Emechebe U, Smith R, Franklin S, Moore B, Yandell M, Lessnick SL, Moon AM
eLife 2014 May 29;3
eLife 2014 May 29;3
Mitochondrial dysfunction in Pten haplo-insufficient mice with social deficits and repetitive behavior: interplay between Pten and p53.
Napoli E, Ross-Inta C, Wong S, Hung C, Fujisawa Y, Sakaguchi D, Angelastro J, Omanska-Klusek A, Schoenfeld R, Giulivi C
PloS one 2012;7(8):e42504
PloS one 2012;7(8):e42504
UV-induced ablation of the epidermal basal layer including p53-mutant clones resets UV carcinogenesis showing squamous cell carcinomas to originate from interfollicular epidermis.
Rebel HG, Bodmann CA, van de Glind GC, de Gruijl FR
Carcinogenesis 2012 Mar;33(3):714-20
Carcinogenesis 2012 Mar;33(3):714-20
The effect of the aqueous extract of the roots of Asparagus racemosus on hepatocarcinogenesis initiated by diethylnitrosamine.
Agrawal A, Sharma M, Rai SK, Singh B, Tiwari M, Chandra R
Phytotherapy research : PTR 2008 Sep;22(9):1175-82
Phytotherapy research : PTR 2008 Sep;22(9):1175-82
Conformationally altered p53: a novel Alzheimer's disease marker?
Lanni C, Racchi M, Mazzini G, Ranzenigo A, Polotti R, Sinforiani E, Olivari L, Barcikowska M, Styczynska M, Kuznicki J, Szybinska A, Govoni S, Memo M, Uberti D
Molecular psychiatry 2008 Jun;13(6):641-7
Molecular psychiatry 2008 Jun;13(6):641-7
Uveal spindle cell tumor of blue-eyed dogs: an immunohistochemical study.
Zarfoss MK, Klauss G, Newkirk K, Kiupel M, Jones Y, Colitz CM, Dubielzig RR
Veterinary pathology 2007 May;44(3):276-84
Veterinary pathology 2007 May;44(3):276-84
A novel loss-of-function mutation in TP53 in an endometrial cancer cell line and uterine papillary serous carcinoma model.
Liu Z, Wan G, Heaphy C, Bisoffi M, Griffith JK, Hu CA
Molecular and cellular biochemistry 2007 Mar;297(1-2):179-87
Molecular and cellular biochemistry 2007 Mar;297(1-2):179-87
Delayed activation of Bax by DNA damage in embryonic stem cells with knock-in mutations of the Abl nuclear localization signals.
Preyer M, Shu CW, Wang JY
Cell death and differentiation 2007 Jun;14(6):1139-48
Cell death and differentiation 2007 Jun;14(6):1139-48
A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers.
Rosas-Acosta G, Russell WK, Deyrieux A, Russell DH, Wilson VG
Molecular & cellular proteomics : MCP 2005 Jan;4(1):56-72
Molecular & cellular proteomics : MCP 2005 Jan;4(1):56-72
The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model.
Mathur S, Kaur P, Sharma M, Katyal A, Singh B, Tiwari M, Chandra R
Phytomedicine : international journal of phytotherapy and phytopharmacology 2004 Jul;11(5):452-60
Phytomedicine : international journal of phytotherapy and phytopharmacology 2004 Jul;11(5):452-60
The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model.
Mathur S, Kaur P, Sharma M, Katyal A, Singh B, Tiwari M, Chandra R
Phytomedicine : international journal of phytotherapy and phytopharmacology 2004 Jul;11(5):452-60
Phytomedicine : international journal of phytotherapy and phytopharmacology 2004 Jul;11(5):452-60
Ionizing radiation-induced apoptosis via separate Pms2- and p53-dependent pathways.
Zeng M, Narayanan L, Xu XS, Prolla TA, Liskay RM, Glazer PM
Cancer research 2000 Sep 1;60(17):4889-93
Cancer research 2000 Sep 1;60(17):4889-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p53 was performed by loading 40 µg of Nuclear protein extract from BEAS-2B in sample buffer and 5µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 10% polyacrylamide gel. Proteins were transferred to PVDF membrane (Product # 88518) at 65V for 90 min on ice. Membrane was blocked in 5% Milk in PBST for 1 hour at room temperature. p53 was detected at approximately 70 kDa using p53 monoclonal antibody (Product # 13-4100) at a dilution of 1:1500 in 0.5% Milk in PBST overnight at 4°C. Followed by goat anti-mouse alkaline phosphatase secondary antibody at a dilution of 1:20,000. Chemiluminescent detection was performed using ECF substrate. Data courtesy of Dr. Fondufe-Mittendorf's lab.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Protein expression of p53, p21 and SCO2 and CCO activity in Pten-deficient striatal neurons. A. Representative Western blot and densitometry of p-Akt and total Akt protein levels in striatal neurons. Results are expressed as the ratio between p-Akt and total Akt and reported as mean +- SEM. B. Representative Western blots of p53, p21 SCO2 and Tubulin. Forty ug of protein were loaded in each lane. Details on the antibodies used are described in the Materials and Methods . C Densitometry results for p53, p21, SCO2 protein expression, normalized by Tubulin (loading control). CCO activity was expressed as nmolx(minx10 6 cells) -1 and reported normalized by citrate synthase. Results are mean +- SEM of experiments in triplicates.
