AHO0112

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

AHO0112

Provider

Invitrogen Antibodies

Product name

p53 Monoclonal Antibody (PAb 240)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with only mutant p53 protein under non-denaturing conditions (Immunoprecipitation) but reacts with both mutant and wild p53 under denaturing conditions (Western blot). It reacts with an epitope at aa 212-217. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls include MDAD-231 cells, SK-BR-3 cells, colon carcinoma, or squamous cell carcinoma.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

PAb 240

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

3-Bromopyruvate inhibits human gastric cancer tumor growth in nude mice via the inhibition of glycolysis.

Xian SL, Cao W, Zhang XD, Lu YF
Oncology letters 2015 Feb;9(2):739-744

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Analysis of the expression of p53 during the morphogenesis of the gastroesophageal mucosa of Gallus gallus domesticus (Linnaeus, 1758).

Ventura A, do Nascimento AA, dos Santos MA, Vieira-Lopes DA, Sales A, Pinheiro NL
Cell and tissue research 2014 Jan;355(1):111-9

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Rapid assessment of RNAi-mediated protein depletion by selected reaction monitoring mass spectrometry.

Glukhova VA, Tomazela DM, Findlay GD, Monnat RJ Jr, MacCoss MJ
Journal of proteome research 2013 Jul 5;12(7):3246-54

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UV-induced ablation of the epidermal basal layer including p53-mutant clones resets UV carcinogenesis showing squamous cell carcinomas to originate from interfollicular epidermis.

Rebel HG, Bodmann CA, van de Glind GC, de Gruijl FR
Carcinogenesis 2012 Mar;33(3):714-20

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The effect of the aqueous extract of the roots of Asparagus racemosus on hepatocarcinogenesis initiated by diethylnitrosamine.

Agrawal A, Sharma M, Rai SK, Singh B, Tiwari M, Chandra R
Phytotherapy research : PTR 2008 Sep;22(9):1175-82

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Delayed activation of Bax by DNA damage in embryonic stem cells with knock-in mutations of the Abl nuclear localization signals.

Preyer M, Shu CW, Wang JY
Cell death and differentiation 2007 Jun;14(6):1139-48

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A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers.

Rosas-Acosta G, Russell WK, Deyrieux A, Russell DH, Wilson VG
Molecular & cellular proteomics : MCP 2005 Jan;4(1):56-72

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The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model.

Mathur S, Kaur P, Sharma M, Katyal A, Singh B, Tiwari M, Chandra R
Phytomedicine : international journal of phytotherapy and phytopharmacology 2004 Jul;11(5):452-60

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The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model.

Mathur S, Kaur P, Sharma M, Katyal A, Singh B, Tiwari M, Chandra R
Phytomedicine : international journal of phytotherapy and phytopharmacology 2004 Jul;11(5):452-60

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Immunohistochemical expression of p53 in animal tumors: a methodological study using four anti-human p53 antibodies.

Albaric O, Bret L, Amardeihl M, Delverdier M
Histology and histopathology 2001 Jan;16(1):113-21

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), COS-7 (Lane 2), A431 (Lane 3), and SKOV3 (Lane 4). The blots were probed with Anti-p53 Mouse Monoclonal Antibody (Product # AHO0112, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 53 kDa band corresponding to p53 was observed across cell lines tested except SKOV3. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p53 in MDA231 cells using a p53 monoclonal antibody (Product # AHO0112).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of p53 in formalin-fixed, paraffin-embedded human colon carcinoma using a p53 monoclonal antibody (Product # AHO0112). Detection was performed with a peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of p53 in denatured human MDA231 cells using a p53 monoclonal antibody (Product # AHO0112).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of p53 in native human SKBR3 cells using a p53 monoclonal antibody (Product # AHO0112).