NB200-104
antibody from Novus Biologicals
Targeting: TP53
LFS1, p53
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NB200-104 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-104, RRID:AB_10001307
- Product name
- Mouse Monoclonal p53 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognizes both wild-type and mutant forms of human p53 protein.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Identification and comparative analyses of myocardial miRNAs involved in the fetal response to maternal obesity.
Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer.
COP1, the negative regulator of p53, is overexpressed in breast and ovarian adenocarcinomas.
Immunohistochemical analysis of p53 expression in malignant lymphomas.
Maloyan A, Muralimanoharan S, Huffman S, Cox LA, Nathanielsz PW, Myatt L, Nijland MJ
Physiological genomics 2013 Oct 1;45(19):889-900
Physiological genomics 2013 Oct 1;45(19):889-900
Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer.
Yamamura S, Kawakami K, Hirata H, Ueno K, Saini S, Majid S, Dahiya R
Molecular cancer therapeutics 2010 Jun;9(6):1680-7
Molecular cancer therapeutics 2010 Jun;9(6):1680-7
COP1, the negative regulator of p53, is overexpressed in breast and ovarian adenocarcinomas.
Dornan D, Bheddah S, Newton K, Ince W, Frantz GD, Dowd P, Koeppen H, Dixit VM, French DM
Cancer research 2004 Oct 15;64(20):7226-30
Cancer research 2004 Oct 15;64(20):7226-30
Immunohistochemical analysis of p53 expression in malignant lymphomas.
Said JW, Barrera R, Shintaku IP, Nakamura H, Koeffler HP
The American journal of pathology 1992 Dec;141(6):1343-8
The American journal of pathology 1992 Dec;141(6):1343-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p53 Antibody (PAb 1801) [NB200-104] - Lanes are as follows:. Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane 5 - 1.5ug of A549 lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p53 Antibody (Pab1801) [NB200-104] - Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (NB200-104). (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by NB200-104. EasyBlot anti-Mouse IgG Kit was used in Western blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p53 Antibody (PAb 1801) [NB200-104] - Tumor tissues from human medulloblastoma cells xenografted in the mouse cerebellum stained with p53 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p53 Antibody (PAb 1801) [NB200-104] - Paraffin-embedded NCl-H1299 xenograft. p53 antibody [Pab1801]) diluted at 1:200.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104] - Immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell Lysate/extract treated with CPT 500nM for 6hr A. 5 ug preimmune mouse IgG B. 5 ug of p53 antibody (NB200-104) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.