ABIN362058

antibody from antibodies-online

Targeting: TP53 LFS1, p53

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362058

Provider

antibodies-online

Product name

anti-Tumor Protein P53 (TP53) (pSer315) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer315 (S-S-S (p) -P-Q) derived from Human p53. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human

Host

Rabbit

Epitope

pSer315

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro.

Lu H, Fisher RP, Bailey P, Levine AJ
Molecular and cellular biology 1997 Oct;17(10):5923-34

---

Differential effects of phosphorylation of rat p53 on transactivation of promoters derived from different p53 responsive genes.

Lohrum M, Scheidtmann KH
Oncogene 1996 Dec 19;13(12):2527-39