Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-17545 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-p53 (Thr81) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-p53 pThr81 in extracts from HT29 cells, untreated, nocodazole-treated (50 ng/mL, 24h) or UV-treated (50mJ/cm2, 1hr), using Phospho-p53 pThr81 polyclonal antibody (Product # PA5-17545) (upper), p53 monoclonal antibody (middle) and Phospho-SAPK/JNK (T183/Y185) monoclonal antibody (lower).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-p53 pThr81 in paraffin-embedded HT-29 cells, untreated (left) or nocodazole-treated (right), using a Phospho-p53 pThr81 polyclonal antibody (Product # PA5-17545) in the presence of control peptide (lower) or antigen-specific peptide (upper).