PA5-17817

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17817

Provider

Invitrogen Antibodies

Product name

Phospho-p53 (Ser46) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with p53 phosphorylated at other sites.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

20 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Phospho-p53 (Ser46) Polyclonal Antibody (Product # PA5-17817) and a 53kDa band corresponding to phospho-Cellular tumor antigen p53 was observed to be up-regulated in HEK-293 cell line upon Doxorubicin treatment. Whole-cell extracts (30 µg lysate) of HEK-293 (Lane 1), HEK-293 treated with Doxorubicin (0.5 micromolar for 24h) (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody ((1:1000 dilution)) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-p53 pSer46 in MCF-7 cells, etoposide-treated, using a Phospho-p53 pSer46 polyclonal antibody (Product # PA5-17817) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Phospho-p53 pSer46 in untreated (blue) or UV-treated (green) HT-29 cells using a Phospho-p53 pSer46 polyclonal antibody (Product # PA5-17817).