PA1-4635

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4635

Provider

Invitrogen Antibodies

Product name

Phospho-p53 (Ser392) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with human and some non-human primates based on 100% sequence homology.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blots of 10 µg of PMA treated NIH 3T3 cell lysate (100 nM for fifteen minutes) showing specific immunolabeling of ~53 kDa p53 phosphorylated at Ser392. The labeling by the antibody was specifically blocked by the Ser392 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of p53 in rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa Phospho-p53 (Ser392) polyclonal antibody (Product # PA1-4635) in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1200 units for 30 minutes).