Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-4635 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-p53 (Ser392) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with human and some non-human primates based on 100% sequence homology. This antibody is specific for the ~53 kDa p53 protein phosphorylated at Ser392 in Western blots of NIH 3T3 cell lysates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blots of 10 µg of PMA treated NIH 3T3 cell lysate (100 nM for fifteen minutes) showing specific immunolabeling of ~53 kDa p53 phosphorylated at Ser392. The labeling by the antibody was specifically blocked by the Ser392 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of p53 in rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa Phospho-p53 (Ser392) polyclonal antibody (Product # PA1-4635) in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1200 units for 30 minutes).
