Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-17287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Acetyl-p53 (Lys379) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 163 µg/mL
- Storage
- -20°C
Submitted references Severe Spinal Cord Injury in Rats Induces Chronic Changes in the Spinal Cord and Cerebral Cortex Metabolism, Adjusted by Thiamine That Improves Locomotor Performance.
Deregulated expression of HDAC9 in B cells promotes development of lymphoproliferative disease and lymphoma in mice.
Boyko A, Tsepkova P, Aleshin V, Artiukhov A, Mkrtchyan G, Ksenofontov A, Baratova L, Ryabov S, Graf A, Bunik V
Frontiers in molecular neuroscience 2021;14:620593
Frontiers in molecular neuroscience 2021;14:620593
Deregulated expression of HDAC9 in B cells promotes development of lymphoproliferative disease and lymphoma in mice.
Gil VS, Bhagat G, Howell L, Zhang J, Kim CH, Stengel S, Vega F, Zelent A, Petrie K
Disease models & mechanisms 2016 Dec 1;9(12):1483-1495
Disease models & mechanisms 2016 Dec 1;9(12):1483-1495
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Acetyl-p53 (Lys379) in extracts from MCF-7 cells, untreated, doxorubicin-treated (0.5 uM, 24 hours) or doxorubicin and HDAC-inhibitor-treated (400 nM, 24 hours), using Acetyl-p53 (Lys379) polyclonal antibody (Product # PA5-17287).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Acetyl-p53 (Lys379) was performed using 70% confluent log phase HeLa cells treated with 0.5uM Doxorubicin and 400nM Trichostatin A for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-p53 (Lys379) Polyclonal Antibody (Product # PA5-17287) at 1:100 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells with negligible expression of Acetyl-p53(Lys 379). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous Acetyl-p53 (Ser379) protein at specific gene loci using Anti-Acetyl-p53 (Ser379) Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Acetyl-p53 (Ser379) Rabbit Polyclonal Antibody (Product # PA5-17287, 4 µl) on sheared chromatin from 2 million Doxorubicin and Trichostatin A-treated HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the promoters of CDKN1A, CMBL, MDM2, HES2 used as positive, and SAT2 satellite repeats used as negative target genes/binding sites. Data is presented as %IP (IP/Input) of the antibody signal versus the negative control IgG using the comparative CT method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7. Eu-HDAC9 tumors display deregulated acetylation of BCL6 and p53. (A) IHC triple-immunostaining using the ABC-TSA method in mouse spleens from Emu-HDAC9 and wild-type tumors, showing HDAC9 (red) expression in conjunction with levels of acetylated (Ac)-BCL6 (green). (B) Immunoblot analysis of Ac-p53 in Eu- HDAC9 and wild-type spleen. GAPDH was used as a loading control. (C) ABC-TSA immunofluorescence analysis of Ac-p53 (green) and HDAC9 (red) in spleen in Emu- HDAC9 and wild-type controls.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 8 Comparison of the effects of the OGDHC regulators thiamine (Th) and TEGP on the expression of sirtuin 5 (A) and total or acetylated p53 (B) in cerebral cortex of the experimental rat groups. The expression is assessed by the western-blotting procedures described in Methods. Data for the different animal groups are expressed as percentage of the corresponding values determined in the non-operated (control, C) rats. The two-way ANOVA and post hoc Tukey''s test are used to determine statistical significance of differences. On the graphs, different p -values are shown by asterisks as described in Methods under Statistical Analysis. In the tables, significances ( p