PA5-97932

antibody from Invitrogen Antibodies

Targeting: LPAR4 GPR23, LPA4, P2RY9, P2Y5-LIKE, P2Y9

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97932

Provider

Invitrogen Antibodies

Product name

LPAR4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

2 mg/mL

Storage

-20°C or -80°C if preferred

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of LPAR4 in HepG2 cells using a LPAR4 polyclonal antibody (Product # PA5-97932) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LPAR4 in paraffin embedded human endometrial cancer using a LPAR4 polyclonal antibody (Product # PA5-97932) at a dilution of 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.