NBP2-01761

antibody from Novus Biologicals

Targeting: IDS ID2S, SIDS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-01761

Provider

Novus Biologicals

Product name

Mouse Monoclonal Iduronate 2-Sulfatase/IDS Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.71 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

FGF signaling deregulation is associated with early developmental skeletal defects in animal models for mucopolysaccharidosis type II (MPSII).

Bellesso S, Salvalaio M, Lualdi S, Tognon E, Costa R, Braghetta P, Giraudo C, Stramare R, Rigon L, Filocamo M, Tomanin R, Moro E
Human molecular genetics 2018 Jul 1;27(13):2262-2275

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Iduronate 2 sulfatase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Iduronate 2 sulfatase.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Iduronate 2 sulfatase monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human prostate tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human lymphoma tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human liver tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human Kidney tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Human tonsil.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Carcinoma of Human liver tissue.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Iduronate 2 sulfatase antibody, and then analyzed by flow cytometry.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of Hela cells, using anti-Iduronate 2 sulfatase antibody, (Red), compared to a nonspecific negative control antibody (Blue).