Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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- Product number
- AF2449 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Iduronate 2-Sulfatase/IDS Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IDS in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IDS is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22304
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dose-Dependent Prevention of Metabolic and Neurologic Disease in Murine MPS II by ZFN-Mediated In Vivo Genome Editing.
Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells.
Localized donor cells in brain of a Hunter disease patient after cord blood stem cell transplantation.
Laoharawee K, DeKelver RC, Podetz-Pedersen KM, Rohde M, Sproul S, Nguyen HO, Nguyen T, St Martin SJ, Ou L, Tom S, Radeke R, Meyer KE, Holmes MC, Whitley CB, Wechsler T, McIvor RS
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Apr 4;26(4):1127-1136
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Apr 4;26(4):1127-1136
Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells.
Kondo Y, Toyoda T, Ito R, Funato M, Hosokawa Y, Matsui S, Sudo T, Nakamura M, Okada C, Zhuang X, Watanabe A, Ohta A, Inagaki N, Osafune K
Diabetologia 2017 Aug;60(8):1454-1466
Diabetologia 2017 Aug;60(8):1454-1466
Localized donor cells in brain of a Hunter disease patient after cord blood stem cell transplantation.
Araya K, Sakai N, Mohri I, Kagitani-Shimono K, Okinaga T, Hashii Y, Ohta H, Nakamichi I, Aozasa K, Taniike M, Ozono K
Molecular genetics and metabolism 2009 Nov;98(3):255-63
Molecular genetics and metabolism 2009 Nov;98(3):255-63
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Iduronate 2-Sulfatase/IDS by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Iduronate 2-Sulfatase/IDS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2449) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Iduronate 2-Sulfatase/IDS at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.