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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX102399 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102399, RRID:AB_1949609
- Product name
- AIF antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Enhanced validation
Supportive validation
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- GeneTex (provider)
- Enhanced method
- Genetic validation
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- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term (GTX102399) diluted at 1:4000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract E. 30 ?g A375 whole cell lysate/extract 7.5 % SDS-PAGEAIF antibody [N1N2], N-term (GTX102399) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term (GTX102399) diluted at 1:4000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500.Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AIF(GTX102399) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer, using AIF(GTX102399) antibody at 1:100 dilution.
