PB9366

antibody from Boster Biological Technology

Targeting: AIFM1 AIF, AUNX1, CMTX4, DFNX5, NAMSD, PDCD8

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB9366

Provider

Boster Biological Technology

Product name

Anti-AIF/AIFM1 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for AIF/AIFM1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. AIF/AIFM1 information: Molecular Weight: 66901 MW; Subcellular Localization: Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region; Tissue Specificity: Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Astaxanthin Protects against Alcoholic Liver Injury via Regulating Mitochondrial Redox Balance and Calcium Homeostasis.

Wang P, Zheng X, Du R, Xu J, Li J, Zhang H, Liang X, Liang H
Journal of agricultural and food chemistry 2023 Dec 13;71(49):19531-19550

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Icariin induces apoptosis in acute promyelocytic leukemia by targeting PIM1.

Zhang H, Li P, Li J, Song T, Wang L, Li E, Wang J, Wang L, Wei N, Wang Z
Pharmacological reports : PR 2017 Dec;69(6):1270-1281

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Protective effects of nigranoic acid on cerebral ischemia-reperfusion injury and its mechanism involving apoptotic signaling pathway.

Feng T, Liu Y, Li C, Li Z
Cell biochemistry and biophysics 2015 Jan;71(1):345-51

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Protection by bicyclol derivatives against acetaminophen-induced acute liver failure in mice and its active mechanism.

Hou Y, Li L, Wei H, Wu L, Tong Y, Wu S, Liu G
Liver international : official journal of the International Association for the Study of the Liver 2008 Nov;28(9):1226-35

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of AIF using anti-AIF antibody (PB9366). AIF was detected in paraffin-embedded section of Mouse Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIF Antibody (PB9366) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image