HPA032078

antibody from Atlas Antibodies

Targeting: GPATCH3 FLJ12455, GPATC3

Western blot (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA032078

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA032078, RRID:AB_10603671

Product name

Anti-GPATCH3

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human GPATCH3, Gene description: G patch domain containing 3, Alternative Gene Names: FLJ12455, GPATC3, Validated applications: WB, IHC, Uniprot ID: Q96I76, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.5 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Whole-Exome Sequencing of Congenital Glaucoma Patients Reveals Hypermorphic Variants in GPATCH3, a New Gene Involved in Ocular and Craniofacial Development

Ferre-Fernández J, Aroca-Aguilar J, Medina-Trillo C, Bonet-Fernández J, Méndez-Hernández C, Morales-Fernández L, Corton M, Cabañero-Valera M, Gut M, Tonda R, Ayuso C, Coca-Prados M, García-Feijoo J, Escribano J
Scientific Reports 2017;7(1)

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and GPATCH3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411801).

Sample type

Human

Protocol

Protocol