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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- MA5-33115 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATF2 Recombinant Rabbit Monoclonal Antibody (3D12)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3D12
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references Butorphanol reduces the neuronal inflammatory response and apoptosis via inhibition of p38/JNK/ATF2/p53 signaling.
Huang Y, Li S, Chen H, Feng L, Yuan W, Han T
Experimental and therapeutic medicine 2022 Mar;23(3):229
Experimental and therapeutic medicine 2022 Mar;23(3):229
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cyclic AMP-dependent transcription factor ATF-2 was performed using 70% confluent log phase FaDu cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ATF2 Recombinant Rabbit Monoclonal Antibody (Product # MA5-33115) at 5µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Effect of butorphanol on LPS-induced decrease in PC12 cell viability and p38/JNK/ATF2/p53 signaling. (A) Chemical structure of butorphanol. (B) The effect of different doses of butorphanol on PC12 cell viability was assessed using a CCK8 assay. (C) The viability of cells treated with butorphanol and LPS was determined using a CCK8 assay. (D) The expression levels of p-p38, p-JNK, p-ATF2, p53, p38, JNK and ATF2 were measured using western blot analysis. *** P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Butorphanol protects cells against the inhibitory effects of LPS on viability via p38/JNK/ATF2/p53 signaling. (A) The protein expression levels of p-p38, p-JNK, p-ATF2, p53, p38, JNK and ATF2 in each group were determined using western blot analysis. (B) Cell viability in each group was assessed using a CCK8 assay. (C) The activity of LDH in each group was measured using assay kits. ** P
