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- Antibody Data
- Antigen structure
- References [45]
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- Product number
- 15606-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15606-1-AP, RRID:AB_2238624
- Product name
- RNASEH1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RNASEH1 antibody (Cat. #15606-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references PAPAS promotes differentiation of mammary epithelial cells and suppresses breast carcinogenesis.
ZFP281 controls transcriptional and epigenetic changes promoting mouse pluripotent state transitions via DNMT3 and TET1.
R-Loop Functions in Brca1 -Associated Mammary Tumorigenesis.
Transcription-replication conflicts underlie sensitivity to PARP inhibitors.
TERRA R-loops connect and protect sister telomeres in mitosis.
The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts.
RNF8 ubiquitylation of XRN2 facilitates R-loop resolution and restrains genomic instability in BRCA1 mutant cells.
Excessive transcription-replication conflicts are a vulnerability of BRCA1-mutant cancers.
The CHCHD2/Sirt1 corepressors involve in G9a-mediated regulation of RNase H1 expression to control R-loop.
MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation.
RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis.
A small molecule antagonist of SMN disrupts the interaction between SMN and RNAP II.
The Combination of Mesyl-Phosphoramidate Inter-Nucleotide Linkages and 2'-O-Methyl in Selected Positions in the Antisense Oligonucleotide Enhances the Performance of RNaseH1 Active PS-ASOs.
METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability.
Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.
DDX18 prevents R-loop-induced DNA damage and genome instability via PARP-1.
Protocol to use RNaseH1-based CRISPR to modulate locus-associated R-loops.
NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides.
Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.
ADAR-mediated RNA editing of DNA:RNA hybrids is required for DNA double strand break repair.
R-loops trigger the release of cytoplasmic ssDNAs leading to chronic inflammation upon DNA damage.
Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.
RTEL1 influences the abundance and localization of TERRA RNA.
The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
Site-specific incorporation of 5'-methyl DNA enhances the therapeutic profile of gapmer ASOs.
RNF168 regulates R-loop resolution and genomic stability in BRCA1/2-deficient tumors.
METTL3 and N6-Methyladenosine Promote Homologous Recombination-Mediated Repair of DSBs by Modulating DNA-RNA Hybrid Accumulation.
Mutual Balance of Histone Deacetylases 1 and 2 and the Acetyl Reader ATAD2 Regulates the Level of Acetylation of Histone H4 on Nascent Chromatin of Human Cells.
Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.
The Antitumor Drugs Trabectedin and Lurbinectedin Induce Transcription-Dependent Replication Stress and Genome Instability.
BRCA1-associated R-loop affects transcription and differentiation in breast luminal epithelial cells.
FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.
Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice.
EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.
Chromatin conformation regulates the coordination between DNA replication and transcription.
Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis.
Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.
Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids.
Two familial ALS proteins function in prevention/repair of transcription-associated DNA damage.
Ribonuclease H1-dependent hepatotoxicity caused by locked nucleic acid-modified gapmer antisense oligonucleotides.
The Fanconi Anemia Pathway Protects Genome Integrity from R-loops.
XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides.
Systematic screening reveals a role for BRCA1 in the response to transcription-associated DNA damage.
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
The yeast and human FACT chromatin-reorganizing complexes solve R-loop-mediated transcription-replication conflicts.
Ren S, Bai F, Schnell V, Stanko C, Ritsch M, Schenk T, Barth E, Marz M, Wang B, Pei XH, Bierhoff H
Cell reports 2024 Jan 23;43(1):113644
Cell reports 2024 Jan 23;43(1):113644
ZFP281 controls transcriptional and epigenetic changes promoting mouse pluripotent state transitions via DNMT3 and TET1.
Huang X, Balmer S, Lyu C, Xiang Y, Malik V, Wang H, Zhang Y, Cai B, Xie W, Hadjantonakis AK, Zhou H, Wang J
Developmental cell 2024 Feb 26;59(4):465-481.e6
Developmental cell 2024 Feb 26;59(4):465-481.e6
R-Loop Functions in Brca1 -Associated Mammary Tumorigenesis.
Chiang HC, Qi L, Mitra P, Hu Y, Li R
bioRxiv : the preprint server for biology 2024 Feb 16;
bioRxiv : the preprint server for biology 2024 Feb 16;
Transcription-replication conflicts underlie sensitivity to PARP inhibitors.
Petropoulos M, Karamichali A, Rossetti GG, Freudenmann A, Iacovino LG, Dionellis VS, Sotiriou SK, Halazonetis TD
Nature 2024 Apr;628(8007):433-441
Nature 2024 Apr;628(8007):433-441
TERRA R-loops connect and protect sister telomeres in mitosis.
Sze S, Bhardwaj A, Fnu P, Azarm K, Mund R, Ring K, Smith S
Cell reports 2023 Oct 31;42(10):113235
Cell reports 2023 Oct 31;42(10):113235
The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts.
Bayona-Feliu A, Herrera-Moyano E, Badra-Fajardo N, Galván-Femenía I, Soler-Oliva ME, Aguilera A
Nature communications 2023 Oct 28;14(1):6890
Nature communications 2023 Oct 28;14(1):6890
RNF8 ubiquitylation of XRN2 facilitates R-loop resolution and restrains genomic instability in BRCA1 mutant cells.
Krishnan R, Lapierre M, Gautreau B, Nixon KCJ, El Ghamrasni S, Patel PS, Hao J, Yerlici VT, Guturi KKN, St-Germain J, Mateo F, Saad A, Algouneh A, Earnshaw R, Shili D, Seitova A, Miller J, Khosraviani N, Penn A, Ho B, Sanchez O, Hande MP, Masson JY, Brown GW, Alaoui-Jamali M, Reynolds JJ, Arrowsmith C, Raught B, Pujana MA, Mekhail K, Stewart GS, Hakem A, Hakem R
Nucleic acids research 2023 Oct 27;51(19):10484-10505
Nucleic acids research 2023 Oct 27;51(19):10484-10505
Excessive transcription-replication conflicts are a vulnerability of BRCA1-mutant cancers.
Patel PS, Algouneh A, Krishnan R, Reynolds JJ, Nixon KCJ, Hao J, Lee J, Feng Y, Fozil C, Stanic M, Yerlici T, Su P, Soares F, Liedtke E, Prive G, Baider GD, Pujana MA, Mekhail K, He HH, Hakem A, Stewart GS, Hakem R
Nucleic acids research 2023 May 22;51(9):4341-4362
Nucleic acids research 2023 May 22;51(9):4341-4362
The CHCHD2/Sirt1 corepressors involve in G9a-mediated regulation of RNase H1 expression to control R-loop.
Li L, Wu Y, Dai K, Wang Q, Ye S, Shi Q, Chen Z, Huang YC, Zhao W, Li L
Cell insight 2023 Aug;2(4):100112
Cell insight 2023 Aug;2(4):100112
MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation.
Jin B, Zhu J, Pan T, Yang Y, Liang L, Zhou Y, Zhang T, Teng Y, Wang Z, Wang X, Tian Q, Guo B, Li H, Chen T
Nucleic acids research 2023 Aug 25;51(15):7951-7971
Nucleic acids research 2023 Aug 25;51(15):7951-7971
RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis.
Liu C, Wang L, Li Y, Guo M, Hu J, Wang T, Li M, Yang Z, Lin R, Xu W, Chen Y, Luo M, Gao F, Chen JY, Sun Q, Liu H, Sun B, Li W
Nucleic acids research 2023 Aug 11;51(14):7357-7375
Nucleic acids research 2023 Aug 11;51(14):7357-7375
A small molecule antagonist of SMN disrupts the interaction between SMN and RNAP II.
Liu Y, Iqbal A, Li W, Ni Z, Wang Y, Ramprasad J, Abraham KJ, Zhang M, Zhao DY, Qin S, Loppnau P, Jiang H, Guo X, Brown PJ, Zhen X, Xu G, Mekhail K, Ji X, Bedford MT, Greenblatt JF, Min J
Nature communications 2022 Sep 16;13(1):5453
Nature communications 2022 Sep 16;13(1):5453
The Combination of Mesyl-Phosphoramidate Inter-Nucleotide Linkages and 2'-O-Methyl in Selected Positions in the Antisense Oligonucleotide Enhances the Performance of RNaseH1 Active PS-ASOs.
Zhang L, Liang XH, De Hoyos CL, Migawa M, Nichols JG, Freestone G, Tian J, Seth PP, Crooke ST
Nucleic acid therapeutics 2022 Oct;32(5):401-411
Nucleic acid therapeutics 2022 Oct;32(5):401-411
METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability.
Chen L, Zhang C, Ma W, Huang J, Zhao Y, Liu H
Nucleic acids research 2022 Nov 11;50(20):11619-11634
Nucleic acids research 2022 Nov 11;50(20):11619-11634
Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.
Marasco LE, Dujardin G, Sousa-Luís R, Liu YH, Stigliano JN, Nomakuchi T, Proudfoot NJ, Krainer AR, Kornblihtt AR
Cell 2022 Jun 9;185(12):2057-2070.e15
Cell 2022 Jun 9;185(12):2057-2070.e15
DDX18 prevents R-loop-induced DNA damage and genome instability via PARP-1.
Lin WL, Chen JK, Wen X, He W, Zarceno GA, Chen Y, Chen S, Paull TT, Liu HW
Cell reports 2022 Jul 19;40(3):111089
Cell reports 2022 Jul 19;40(3):111089
Protocol to use RNaseH1-based CRISPR to modulate locus-associated R-loops.
Khosraviani N, Abraham KJ, Chan JNY, Mekhail K
STAR protocols 2022 Dec 16;3(4):101734
STAR protocols 2022 Dec 16;3(4):101734
NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides.
Zhang L, Bernardo KD, Vickers TA, Tian J, Liang XH, Crooke ST
Nucleic acid therapeutics 2022 Aug;32(4):280-299
Nucleic acid therapeutics 2022 Aug;32(4):280-299
Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.
Wu J, Wu C, Xing F, Cao L, Zeng W, Guo L, Li P, Zhong Y, Jiang H, Luo M, Shi G, Bu L, Ji Y, Hou P, Peng H, Huang J, Li C, Guo D
Cell research 2021 Sep;31(9):998-1010
Cell research 2021 Sep;31(9):998-1010
ADAR-mediated RNA editing of DNA:RNA hybrids is required for DNA double strand break repair.
Jimeno S, Prados-Carvajal R, Fernández-Ávila MJ, Silva S, Silvestris DA, Endara-Coll M, Rodríguez-Real G, Domingo-Prim J, Mejías-Navarro F, Romero-Franco A, Jimeno-González S, Barroso S, Cesarini V, Aguilera A, Gallo A, Visa N, Huertas P
Nature communications 2021 Sep 17;12(1):5512
Nature communications 2021 Sep 17;12(1):5512
R-loops trigger the release of cytoplasmic ssDNAs leading to chronic inflammation upon DNA damage.
Chatzidoukaki O, Stratigi K, Goulielmaki E, Niotis G, Akalestou-Clocher A, Gkirtzimanaki K, Zafeiropoulos A, Altmüller J, Topalis P, Garinis GA
Science advances 2021 Nov 19;7(47):eabj5769
Science advances 2021 Nov 19;7(47):eabj5769
Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.
Asada K, Sakaue F, Nagata T, Zhang JC, Yoshida-Tanaka K, Abe A, Nawa M, Nishina K, Yokota T
Nucleic acids research 2021 May 21;49(9):4864-4876
Nucleic acids research 2021 May 21;49(9):4864-4876
RTEL1 influences the abundance and localization of TERRA RNA.
Ghisays F, Garzia A, Wang H, Canasto-Chibuque C, Hohl M, Savage SA, Tuschl T, Petrini JHJ
Nature communications 2021 May 21;12(1):3016
Nature communications 2021 May 21;12(1):3016
The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
Jiang M, Xie X, Zhu X, Jiang S, Milenkovic D, Misic J, Shi Y, Tandukar N, Li X, Atanassov I, Jenninger L, Hoberg E, Albarran-Gutierrez S, Szilagyi Z, Macao B, Siira SJ, Carelli V, Griffith JD, Gustafsson CM, Nicholls TJ, Filipovska A, Larsson NG, Falkenberg M
Science advances 2021 Jul;7(27)
Science advances 2021 Jul;7(27)
Site-specific incorporation of 5'-methyl DNA enhances the therapeutic profile of gapmer ASOs.
Vasquez G, Freestone GC, Wan WB, Low A, De Hoyos CL, Yu J, Prakash TP, Ǿstergaard ME, Liang XH, Crooke ST, Swayze EE, Migawa MT, Seth PP
Nucleic acids research 2021 Feb 26;49(4):1828-1839
Nucleic acids research 2021 Feb 26;49(4):1828-1839
RNF168 regulates R-loop resolution and genomic stability in BRCA1/2-deficient tumors.
Patel PS, Abraham KJ, Guturi KKN, Halaby MJ, Khan Z, Palomero L, Ho B, Duan S, St-Germain J, Algouneh A, Mateo F, El Ghamrasni S, Barbour H, Barnes DR, Beesley J, Sanchez O, Berman HK, Brown GW, Affar EB, Chenevix-Trench G, Antoniou AC, Arrowsmith CH, Raught B, Pujana MA, Mekhail K, Hakem A, Hakem R
The Journal of clinical investigation 2021 Feb 1;131(3)
The Journal of clinical investigation 2021 Feb 1;131(3)
METTL3 and N6-Methyladenosine Promote Homologous Recombination-Mediated Repair of DSBs by Modulating DNA-RNA Hybrid Accumulation.
Zhang C, Chen L, Peng D, Jiang A, He Y, Zeng Y, Xie C, Zhou H, Luo X, Liu H, Chen L, Ren J, Wang W, Zhao Y
Molecular cell 2020 Aug 6;79(3):425-442.e7
Molecular cell 2020 Aug 6;79(3):425-442.e7
Mutual Balance of Histone Deacetylases 1 and 2 and the Acetyl Reader ATAD2 Regulates the Level of Acetylation of Histone H4 on Nascent Chromatin of Human Cells.
Lazarchuk P, Hernandez-Villanueva J, Pavlova MN, Federation A, MacCoss M, Sidorova JM
Molecular and cellular biology 2020 Apr 13;40(9)
Molecular and cellular biology 2020 Apr 13;40(9)
Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.
Deasy SK, Uehara R, Vodnala SK, Yang HH, Dass RA, Hu Y, Lee MP, Crouch RJ, Hunter KW
PLoS genetics 2019 May;15(5):e1008020
PLoS genetics 2019 May;15(5):e1008020
The Antitumor Drugs Trabectedin and Lurbinectedin Induce Transcription-Dependent Replication Stress and Genome Instability.
Tumini E, Herrera-Moyano E, San Martín-Alonso M, Barroso S, Galmarini CM, Aguilera A
Molecular cancer research : MCR 2019 Mar;17(3):773-782
Molecular cancer research : MCR 2019 Mar;17(3):773-782
BRCA1-associated R-loop affects transcription and differentiation in breast luminal epithelial cells.
Chiang HC, Zhang X, Li J, Zhao X, Chen J, Wang HT, Jatoi I, Brenner A, Hu Y, Li R
Nucleic acids research 2019 Jun 4;47(10):5086-5099
Nucleic acids research 2019 Jun 4;47(10):5086-5099
FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.
Pan X, Chen Y, Biju B, Ahmed N, Kong J, Goldenberg M, Huang J, Mohan N, Klosek S, Parsa K, Guh CY, Lu R, Pickett HA, Chu HP, Zhang D
Scientific reports 2019 Dec 13;9(1):19110
Scientific reports 2019 Dec 13;9(1):19110
Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice.
Bhatia V, Valdés-Sánchez L, Rodriguez-Martinez D, Bhattacharya SS
F1000Research 2018;7:1233
F1000Research 2018;7:1233
EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.
Gorthi A, Romero JC, Loranc E, Cao L, Lawrence LA, Goodale E, Iniguez AB, Bernard X, Masamsetti VP, Roston S, Lawlor ER, Toretsky JA, Stegmaier K, Lessnick SL, Chen Y, Bishop AJR
Nature 2018 Mar 15;555(7696):387-391
Nature 2018 Mar 15;555(7696):387-391
Chromatin conformation regulates the coordination between DNA replication and transcription.
Almeida R, Fernández-Justel JM, Santa-María C, Cadoret JC, Cano-Aroca L, Lombraña R, Herranz G, Agresti A, Gómez M
Nature communications 2018 Apr 23;9(1):1590
Nature communications 2018 Apr 23;9(1):1590
Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis.
Zhang X, Chiang HC, Wang Y, Zhang C, Smith S, Zhao X, Nair SJ, Michalek J, Jatoi I, Lautner M, Oliver B, Wang H, Petit A, Soler T, Brunet J, Mateo F, Angel Pujana M, Poggi E, Chaldekas K, Isaacs C, Peshkin BN, Ochoa O, Chedin F, Theoharis C, Sun LZ, Curiel TJ, Elledge R, Jin VX, Hu Y, Li R
Nature communications 2017 Jun 26;8:15908
Nature communications 2017 Jun 26;8:15908
Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.
Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, Cabrini M, Wang Y, Capozzo I, Iannelli F, Matti V, Francia S, Shivashankar GV, Walter NG, d'Adda di Fagagna F
Nature cell biology 2017 Dec;19(12):1400-1411
Nature cell biology 2017 Dec;19(12):1400-1411
Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids.
Abraham KJ, Chan JN, Salvi JS, Ho B, Hall A, Vidya E, Guo R, Killackey SA, Liu N, Lee JE, Brown GW, Mekhail K
Nucleic acids research 2016 Oct 14;44(18):8870-8884
Nucleic acids research 2016 Oct 14;44(18):8870-8884
Two familial ALS proteins function in prevention/repair of transcription-associated DNA damage.
Hill SJ, Mordes DA, Cameron LA, Neuberg DS, Landini S, Eggan K, Livingston DM
Proceedings of the National Academy of Sciences of the United States of America 2016 Nov 29;113(48):E7701-E7709
Proceedings of the National Academy of Sciences of the United States of America 2016 Nov 29;113(48):E7701-E7709
Ribonuclease H1-dependent hepatotoxicity caused by locked nucleic acid-modified gapmer antisense oligonucleotides.
Kasuya T, Hori S, Watanabe A, Nakajima M, Gahara Y, Rokushima M, Yanagimoto T, Kugimiya A
Scientific reports 2016 Jul 27;6:30377
Scientific reports 2016 Jul 27;6:30377
The Fanconi Anemia Pathway Protects Genome Integrity from R-loops.
García-Rubio ML, Pérez-Calero C, Barroso SI, Tumini E, Herrera-Moyano E, Rosado IV, Aguilera A
PLoS genetics 2015 Nov;11(11):e1005674
PLoS genetics 2015 Nov;11(11):e1005674
XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides.
Hori S, Yamamoto T, Obika S
Biochemical and biophysical research communications 2015 Aug 21;464(2):506-11
Biochemical and biophysical research communications 2015 Aug 21;464(2):506-11
Systematic screening reveals a role for BRCA1 in the response to transcription-associated DNA damage.
Hill SJ, Rolland T, Adelmant G, Xia X, Owen MS, Dricot A, Zack TI, Sahni N, Jacob Y, Hao T, McKinney KM, Clark AP, Reyon D, Tsai SQ, Joung JK, Beroukhim R, Marto JA, Vidal M, Gaudet S, Hill DE, Livingston DM
Genes & development 2014 Sep 1;28(17):1957-75
Genes & development 2014 Sep 1;28(17):1957-75
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
Bhatia V, Barroso SI, García-Rubio ML, Tumini E, Herrera-Moyano E, Aguilera A
Nature 2014 Jul 17;511(7509):362-5
Nature 2014 Jul 17;511(7509):362-5
The yeast and human FACT chromatin-reorganizing complexes solve R-loop-mediated transcription-replication conflicts.
Herrera-Moyano E, Mergui X, García-Rubio ML, Barroso S, Aguilera A
Genes & development 2014 Apr 1;28(7):735-48
Genes & development 2014 Apr 1;28(7):735-48
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The RNASEH1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RNASEH1. This antibody recognizes human, mouse, rat antigen. The RNASEH1 antibody has been validated for the following applications: ELISA, WB analysis.
