PB9165

antibody from Boster Biological Technology

Targeting: CAV1 CAV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB9165

Provider

Boster Biological Technology

Product name

Anti-Caveolin-1/CAV1 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CAVEOLIN 1/CAV1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC, IF, FCM. Reactive species: Human. CAVEOLIN 1/CAV1 information: Molecular Weight: 20472 MW; Subcellular Localization: Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin- like structure in the membrane. Membrane protein of caveolae; Tissue Specificity: Expressed in muscle and lung, less so in liver, brain and kidney.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

LncRNA IMFlnc1 promotes porcine intramuscular adipocyte adipogenesis by sponging miR-199a-5p to up-regulate CAV-1.

Wang J, Chen MY, Chen JF, Ren QL, Zhang JQ, Cao H, Xing BS, Pan CY
BMC molecular and cell biology 2020 Nov 4;21(1):77

---

Involvement of CGRP-RCP in the caveolin-1/ERK1/2 signal pathway in the static pressure-induced proliferation of vascular smooth muscle cells.

Guo F, Yang L, Luo J, Quan H, Wang Z, Peng H, Hong C, Li J, Jiang Z, Zhang L, Qin X
Journal of cellular physiology 2018 Oct;233(10):6910-6920

---

Impacts of Dietary Pleurotus eryngii Polysaccharide on Nutrient Digestion, Metabolism, and Immune Response of the Small Intestine and Colon-An iTRAQ-Based Proteomic Analysis.

Ma G, Kimatu BM, Zhao L, Yang W, Pei F, Hu Q
Proteomics 2018 Apr;18(7):e1700443

---

Mechanistic analysis of taxol-induced multidrug resistance in an ovarian cancer cell line.

Wang NN, Zhao LJ, Wu LN, He MF, Qu JW, Zhao YB, Zhao WZ, Li JS, Wang JH
Asian Pacific journal of cancer prevention : APJCP 2013;14(9):4983-8

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Caveolin-1 using anti-Caveolin-1 antibody (PB9165). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human Caveolin-1 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-1 antigen affinity purified polyclonal antibody (Catalog # PB9165) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-1 at approximately 40KD. The expected band size for Caveolin-1 is at 40KD.

Additional image