GTX109250

GSTT1 antibody from GeneTex

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

Immunoprecipitation

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

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Antibody Data

Product number

GTX109250

Provider

GeneTex

Proper citation

GeneTex Cat#GTX109250, RRID:AB_1950469

Product name

GSTT1 antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant fragment corresponding to a region within amino acids 1 and 240 of GSTT1 (Uniprot ID#P30711)

Host

Rabbit

Isotype

IgG

Vial size

25 µl, 100 µl

Concentration

1 mg/ml

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References

A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival.
Zhou T, Kim TW, Chong CN, Tan L, Amin S, Sadat Badieyan Z, Mukherjee S, Ghazizadeh Z, Zeng H, Guo M, Crespo M, Zhang T, Kenyon R, Robinson CL, Apostolou E, Wang H, Xiang JZ, Evans T, Studer L, Chen S
Nature communications 2018 Nov 16;9(1):4815

Western blot

Supportive validation

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GeneTex (provider)

Main image

Experimental details

GSTT1 antibody detects GSTT1 protein by western blot analysis.A. 50 ?g rat liver lysate/extract12% SDS-PAGEGSTT1 antibody (GTX109250) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate)A: HepG2 (GTX27900)10% SDS PAGEGTX109250 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GSTT1 antibody detects GSTT1 protein by western blot analysis.A. 50 ?g mouse spleen lysate/extract12% SDS-PAGEGSTT1 antibody (GTX109250) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 £gg of GSTT1 antibody (GTX109250).Western blot analysis was performed using GSTT1 antibody (GTX109250).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GSTT1 antibody detects GSTT1 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. GSTT1 antibody (GTX109250) dilution: 1:500.