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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX109250 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109250, RRID:AB_1950469
- Product name
- GSTT1 antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant fragment corresponding to a region within amino acids 1 and 240 of GSTT1 (Uniprot ID#P30711)
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl, 100 µl
- Concentration
- 1 mg/ml
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Submitted references A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival.
Zhou T, Kim TW, Chong CN, Tan L, Amin S, Sadat Badieyan Z, Mukherjee S, Ghazizadeh Z, Zeng H, Guo M, Crespo M, Zhang T, Kenyon R, Robinson CL, Apostolou E, Wang H, Xiang JZ, Evans T, Studer L, Chen S
Nature communications 2018 Nov 16;9(1):4815
Nature communications 2018 Nov 16;9(1):4815
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GSTT1 antibody detects GSTT1 protein by western blot analysis.A. 50 ?g rat liver lysate/extract12% SDS-PAGEGSTT1 antibody (GTX109250) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: HepG2 (GTX27900)10% SDS PAGEGTX109250 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GSTT1 antibody detects GSTT1 protein by western blot analysis.A. 50 ?g mouse spleen lysate/extract12% SDS-PAGEGSTT1 antibody (GTX109250) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 £gg of GSTT1 antibody (GTX109250).Western blot analysis was performed using GSTT1 antibody (GTX109250).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GSTT1 antibody detects GSTT1 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. GSTT1 antibody (GTX109250) dilution: 1:500.
