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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-22011 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GSTT1
- Antibody type
- Polyclonal
- Antigen
- Recombinant fragment corresponding to a region within amino acids 1 and 240 of GSTT1 (Uniprot ID#P30711)
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSTT1 using 50 µg mouse spleen lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a GSTT1 polyclonal antibody (Product # PA5-22011) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSTT1 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GSTT1 polyclonal antibody (Product # PA5-22011) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTT1 Polyclonal Antibody detects GSTT1 protein by western blot analysis. A. 50 µg rat liver lysate/extract.12% SDS-PAGE. GSTT1 Polyclonal Antibody (Product # PA5-22011) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTT1 Polyclonal Antibody detects GSTT1 protein by Western blot analysis. Mouse tissue extracts (50 µg) was separated by 12 % SDS-PAGE, and the membrane was blotted with GSTT1 Polyclonal Antibody (Product # PA5-22011) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTT1 Polyclonal Antibody detects GSTT1 protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTT1 Polyclonal Antibody (Product # PA5-22011) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTT1 Polyclonal Antibody detects GSTT1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. GSTT1 Polyclonal Antibody (Product # PA5-22011) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GSTT1 was performed in HepG2 whole cell extracts using 5 µg of GSTT1 Polyclonal Antibody (Product # PA5-22011). Samples were transferred to a membrane and probed with GSTT1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
