NBP1-32733

antibody from Novus Biologicals

Targeting: GSTT1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-32733

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-32733, RRID:AB_2295174

Product name

Rabbit Polyclonal GSTT1 Antibody

Antibody type

Polyclonal

Antigen

Recombinant fragment corresponding to a region within amino acids 1 and 240 of GSTT1 (Uniprot ID#P30711)

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Aliquot and store at -20° or -80°. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GSTT1 Antibody [NBP1-32733] - Sample (30 ug of whole cell lysate)A: Hep G2 10% SDS PAGE, antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GSTT1 Antibody [NBP1-32733] - A. 50 ug mouse spleen lysate/extract 12 % SDS-PAGE GSTT1 antibody dilution: 1:500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GSTT1 Antibody [NBP1-32733] - A. 50 ug rat liver lysate/extract 12 % SDS-PAGE GSTT1 antibody dilution: 1:500

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: GSTT1 Antibody [NBP1-32733] - Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 ug of GSTT1 antibody. Western blot analysis was performed using GSTT1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GSTT1 Antibody [NBP1-32733] - Human hepatoma. GSTT1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: GSTT1 Antibody [NBP1-32733] - Analysis of paraffin-embedded SW480 xenograft, using GSTT1 antibody at 1:500 dilution.