Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-32733 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32733, RRID:AB_2295174
- Product name
- Rabbit Polyclonal GSTT1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant fragment corresponding to a region within amino acids 1 and 240 of GSTT1 (Uniprot ID#P30711)
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20° or -80°. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: GSTT1 Antibody [NBP1-32733] - Sample (30 ug of whole cell lysate)A: Hep G2 10% SDS PAGE, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GSTT1 Antibody [NBP1-32733] - A. 50 ug mouse spleen lysate/extract 12 % SDS-PAGE GSTT1 antibody dilution: 1:500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GSTT1 Antibody [NBP1-32733] - A. 50 ug rat liver lysate/extract 12 % SDS-PAGE GSTT1 antibody dilution: 1:500
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GSTT1 Antibody [NBP1-32733] - Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 ug of GSTT1 antibody. Western blot analysis was performed using GSTT1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GSTT1 Antibody [NBP1-32733] - Human hepatoma. GSTT1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GSTT1 Antibody [NBP1-32733] - Analysis of paraffin-embedded SW480 xenograft, using GSTT1 antibody at 1:500 dilution.