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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
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- Product number
- NB200-541 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-541, RRID:AB_10001380
- Product name
- Rat Monoclonal Complement C4b/d Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Antibody cross reacts with C4, C4b and C4d.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Anti-TCRβ mAb induces long-term allograft survival by reducing antigen-reactive T cells and sparing regulatory T cells.
Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants.
Allograft rejection requires STAT5a/b-regulated antiapoptotic activity in T cells but not B cells.
Anti-DNA autoreactivity in C4-deficient mice.
Macrophage-derived complement component C4 can restore humoral immunity in C4-deficient mice.
Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4.
Miyahara Y, Khattar M, Schroder PM, Mierzejewska B, Deng R, Han R, Hancock WW, Chen W, Stepkowski SM
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Jun;12(6):1409-18
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Jun;12(6):1409-18
Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants.
Murata K, Fox-Talbot K, Qian Z, Takahashi K, Stahl GL, Baldwin WM 3rd, Wasowska BA
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2007 Nov;7(11):2605-14
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2007 Nov;7(11):2605-14
Allograft rejection requires STAT5a/b-regulated antiapoptotic activity in T cells but not B cells.
Zhang Y, Kirken RA, Furian L, Janczewska S, Qu X, Hancock WW, Wang M, Tejpal N, Kerman R, Kahan BD, Stepkowski SM
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan 1;176(1):128-37
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan 1;176(1):128-37
Anti-DNA autoreactivity in C4-deficient mice.
Paul E, Pozdnyakova OO, Mitchell E, Carroll MC
European journal of immunology 2002 Sep;32(9):2672-9
European journal of immunology 2002 Sep;32(9):2672-9
Macrophage-derived complement component C4 can restore humoral immunity in C4-deficient mice.
Gadjeva M, Verschoor A, Brockman MA, Jezak H, Shen LM, Knipe DM, Carroll MC
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5489-95
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5489-95
Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4.
Kremmer E, Thierfelder S, Felber E, Hoffmann-Fezer G, Wasiliu M
Hybridoma 1990 Aug;9(4):309-17
Hybridoma 1990 Aug;9(4):309-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.
