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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP4998a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP4998a, RRID:AB_10664016
- Product name
- CCR7 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "CCR7 Antibody (N-term) (Cat. #AP4998a) western blot analysis in 293(lane 1),Ramos(lane 2),MDA-MB231(lane 3) cell line and mouse spleen tissue(lane 4) lysates (35ug/lane).This demonstrates the CCR7 antibody detected the CCR7 protein (arrow)."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CCR7 Antibody (N-term)(Cat#AP4998a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- CCR7 Antibody (N-term) (Cat. #AP4998a) IHC analysis in formalin fixed and paraffin embedded human tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CCR7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- CCR7 Antibody (N-term) (Cat. #AP4998a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
