Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA303434 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA303434, RRID:AB_2075156
- Product name
- Rabbit monoclonal antibody against Cdc25C(E302)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against Cdc25C(E302)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CDC25C
- Isotype
- IgG
- Antibody clone number
- E302
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - Cdc25C antibody [E302]; Anti-Cdc25C antibody [E302] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 53 kDa.Observed band size : 60 kDa .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin-embedded sections) - Cdc25C antibody [E302]; Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma TA303434 at 1/250 dilution.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry - Anti-Cdc25C antibody [E302]; Flow cytometry analysis of HeLa cells, staining Cdc25C with TA303434. Cells were fixed with formaldehyde and permeabilized with 90% methanol. Samples were incubated with primary antibody (1/20 in PBS + 10% goat serum) for 1 hour at 23°C. A FITC-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.
- Validation comment
- FC
