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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- MAB0007 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0007, RRID:AB_628547
- Product name
- CDC25C (phospho S216) monoclonal antibody, clone TK-1F1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic phosphopeptide of CDC25C.
- Isotype
- IgG
- Antibody clone number
- TK-1F1
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- In vitro kinase reaction and detection of phosphorylation of Cdc25C at Ser216 residue by using Cdc25C (phospho S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007). GST-Cdc25C was phosphorylated by Checkpoint kinase like as Chk1 and proved by Cdc25C (phospho-S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007) after SDS-PAGE and transfer to PVDF membrane.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA for measurement of recombinant Chk1 and Chk2 activity using Cdc25C (phospho-S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007).
- Validation comment
- ELISA
