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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00000995-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000995-D01, RRID:AB_10633504
- Product name
- CDC25C MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human CDC25C protein.
- Antigen sequence
MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLER
DTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKC
CLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQ
EVHLAGMNHDQHLMKCSPAQLLCSTPNGLDRGHRK
RDAMCSSSANKENDNGNLVDSEMKYLGSPITTVPK
LDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSG
LYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKY
FSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQG
HLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLS
GKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQ
EELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGP
RMCRCLREEDRSLNQYPALYYPELYILKGGYRDFF
PEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQE
GERQLREQIALLVKDMSP- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CDC25C expression in transfected 293T cell line (H00000995-T01) by CDC25C MaxPab polyclonal antibody.Lane 1: CDC25C transfected lysate(53.30 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of CDC25C transfected lysate using anti-CDC25C MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CDC25C MaxPab mouse polyclonal antibody (B01) (H00000995-B01).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
