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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00000995-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000995-M01, RRID:AB_537610
- Product name
- Mouse Monoclonal CDC25C Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CDC25C - cell division cycle 25C
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi ( Cat # H00000995-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody (M01), clone 3B11 (Cat # H00000995-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.Lane 1: CDC25C transfected lysate(53.312 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - CDC25C monoclonal antibody (M01), clone 3B11 Analysis of CDC25C expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CDC25C Antibody (3B11) [H00000995-M01] - Detection limit for recombinant GST tagged CDC25C is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDC25C MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 6 ug/ml.
