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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 16485-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDC25C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSTELFSST REEGSSGSGP SFRSNQRKML NLLLERDTSF TVCPDVPRTP VGKFLGDSAN LSILSGGTPK CCLDLSNLSS GEITATQLTT SADLDETGHL DSSGLQEVHL AGMNHDQHLM KCSPAQLLCS TPNGLDRGHR KRDAMCSSSA NKENDNGNLV DSEMKYLGSP ITTVPKLDKN PNLGEDQAEE ISDELMEFSL KDQEAKVSRS GLYRSPSMPE NLNRPRLKQV EKFKDNTIPD KVKKKYFSGQ GKLRKGLCLK KTVSLCDITI TQMLEEDSNQ (1-279 aa encoded by BC019089)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of CDC25C antibody (16485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC25C transfected HeLa cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were intracellularly stained with 0.2 µg Anti-Human CDC25C (Product # 16485-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CDC25C (IP:16485-1-AP, 4ug; Detection:16485-1-AP 1:1000) with K-562 cells lysate 3600ug.
