MA5-14107

antibody from Invitrogen Antibodies

Targeting: CDC25C CDC25, PPP1R60

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14107

Provider

Invitrogen Antibodies

Product name

Cdc25C Monoclonal Antibody (25C14 (TC-14))

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-14107 targets CDC25C in IF, IP, ChIP and WB applications and shows reactivity with Human samples. The MA5-14107 immunogen is recombinant human CDC25C protein.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

25C14 (TC-14)

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

HYAL1 hyaluronidase in prostate cancer: a tumor promoter and suppressor.

Lokeshwar VB, Cerwinka WH, Isoyama T, Lokeshwar BL
Cancer research 2005 Sep 1;65(17):7782-9

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of CDC25C was performed using cross-linked chromatin from 1x10^6 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of a CDC25C monoclonal antibody (Product # MA5-14107). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon 3-3 of human Bcl-x (hBcl-x) or exon 1 of human NR4A3 (hNR4A3). PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. Schematic representations of the Bcl-x and NR4A3 loci are shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions). Regions amplified by Bcl-x and NR4A3 primers are represented by black bars. Data courtesy of the Innovators Program.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CDC25C using CDC25C Monoclonal Antibody (Product # MA5-14107) on denatured Human HeLa Cells.