MA5-15146

antibody from Invitrogen Antibodies

Targeting: CDC25C CDC25, PPP1R60

Immunoprecipitation

Antibody data

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Validation data

CDC25C protein structure - MA5-15146 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Cdc25C pSer216 in HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-Cdc25C pSer216 monoclonal antibody (Product # MA5-15146) (upper) or a cdc25C monoclonal antibody (lower).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Cdc25C pSer216 in GST-cdc25C, unphosphorylated or phosphorylated in vitro by Chk2 using Phospho-Cdc25C pSer216 monoclonal antibody (Product # MA5-15146) (upper) or a cdc25C antibody (lower).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Cdc25C (Ser216) using a monoclonal antibody (Product # MA5-15146).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Phospho-Cdc25C (Ser216) Monoclonal Antibody (K.827.0) (Product # MA5-15146) and a 55 kDa band corresponding to Phospho-Cdc25C (Ser216) was observed in HeLa cells (without phosphatase treatment). Whole Cell Extract (30 µg lysate) of HeLa (Lane 1) and Membrane with HeLa (Lane 2) was treated with Lambda phosphatase (5u/ml, for 16hrs at 4°C). Lysates were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).