Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- MAB10514 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10514, RRID:AB_11188077
- Product name
- JUN monoclonal antibody, clone 5B1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant JUN.
- Isotype
- IgG
- Antibody clone number
- 5B1
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) against NIH/3T3 (1) and COS-7 (2) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) against recombinant protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA detection with JUN monoclonal antibody, clone 5B1 (Cat # MAB10514).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PC-2 cells using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human intima canncer (A) and human rectum cancer (B) tissues using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) (blue) and negative control (red).
- Validation comment
- Flow Cytometry