AP12221PU-N

antibody from Acris Antibodies GmbH

Targeting: JUN AP-1, c-Jun

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP12221PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP12221PU-N, RRID:AB_1748526

Product name

anti AP-1 / c-Jun (Sumoylation Site)

Antibody type

Polyclonal

Antigen

This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide HPRLQALKEEPQTVPE containing a predicted sumoylation site from the C-terminal region of human AP-1.

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

0.25 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

(LEFT) The AP-1 C-term polyclonal antibody (Cat.#AP12221PU-N) is used in Western blot to detect nonsumoylated AP-1 in Jurkat cell lysate. (RIGHT) Western blot analysis of AP-1(arrow) using rabbit polyclonal AP-1 Antibody (C-term) (Cat#AP12221PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AP-1 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.