TA500842

antibody from OriGene

Targeting: JUN AP-1, c-Jun

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500842

Provider

OriGene

Proper citation

OriGene Cat#TA500842, RRID:AB_11125889

Product name

JUN (c-Jun ) mouse monoclonal antibody, clone OTI3G2 (formerly 3G2)

Antibody type

Monoclonal

Description

JUN (c-Jun ) mouse monoclonal antibody, clone OTI3G2 (formerly 3G2)

Host

Mouse

Conjugate

Unconjugated

Epitope

JUN

Isotype

IgG

Antibody clone number

OTI3G2

Vial size

100 µl

Concentration

0.9 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JUN (RC209804, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JUN.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-JUN mouse monoclonal antibody (TA500842) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY JUN(RC209804 ).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC209804 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-JUN antibody(TA500842), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-JUN antibody(TA500842),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC