P02038

antibody from Boster Biological Technology

Targeting: JUN AP-1, c-Jun

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

P02038

Provider

Boster Biological Technology

Product name

Anti-Phospho-c-Jun (S63) Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for C JUN/JUN detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat C JUN/JUN information: Molecular Weight: 35676 MW; Subcellular Localization: Nucleus.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

IGA-10

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Phospho-c-Jun (S63) expression in NIH/3T3 cell lysate treated with Anisomycin (P02038). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Phospho-c-Jun (S63) monoclonal antibody (Catalog # P02038) overnight at 4

Additional image