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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C774649 - Provider product page
- Provider
- LSBio
- Product name
- JUN / c-Jun Antibody (Biotin) LS-C774649
- Antibody type
- Polyclonal
- Description
- Peptide Affinity Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Storage
- Store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using Rabbit Anti-c-Jun Polyclonal Antibody. Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Localization: Nucleus. Magnification: 10X. HRP-DAB Detection.
