MA5-15881

antibody from Invitrogen Antibodies

Targeting: JUN AP-1, c-Jun

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15881

Provider

Invitrogen Antibodies

Product name

c-Jun Monoclonal Antibody (5B1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15881 targets c-Jun in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples. The MA5-15881 immunogen is purified recombinant fragment of human c-Jun expressed in E. Coli. . MA5-15881 detects c-Jun which has a predicted molecular weight of approximately 43kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

5B1

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Cao YJ, Li HZ, Zhao J, Sun YM, Jin XW, Lv SQ, Luo JY, Fang XX, Wen WB, Liao JB
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2264394

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Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.

Deng Y, Miki Y, Nakanishi A
Scientific reports 2020 Jan 28;10(1):1386

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C6orf106 is a novel inhibitor of the interferon-regulatory factor 3-dependent innate antiviral response.

Ambrose RL, Liu YC, Adams TE, Bean AGD, Stewart CR
The Journal of biological chemistry 2018 Jul 6;293(27):10561-10573

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PC-2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (Green). Red: actin filaments have been labeled with phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (blue) and negative control (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (blue) and negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Analysis of E2 signal transduction. ( a ) cAMP assay showing cAMP levels (nM) in MCF-10A cells following treatment with 32 nM E2 for 15 min, 30 min, 24 h, and 48 h. Three independent experiments were performed. Bars represent +/-SD. ( b ) Western blotting of MCF-10A cells showing p38 and phospho-p38 (Thr180/Tyr182) following treatment with 32 nM E2 for 0-60 min. ( c ) Western blotting of MCF-10A cells treated with 32 nM E2 (left panel) or with 32 nM E2 and 20 nM G-15 (right panel) for 0-30 min. ( d ) Western blotting of MCF-10A cells showing JNK and phosphor-JNK (Thr183/Tyr185) following treatment with 32 nM E2 for 0-60 min. ( e ) Western blotting of MCF-10A cells treated with 32 nM E2 (left panel) or with 32 nM E2 and 20 nM G-15 (right panel) for 0-30 min. ( f ) Western blotting of MCF-10A cells treated with 32 nM E2 for 0-60 min showing IkB and phospho-IkB (Ser32, Ser36). ( g ) Western blotting of MCF-10A cells treated with 32 nM E2 for 0-60 min showing c-Jun and phospho-c-Jun (Ser63). ( h ) Representative confocal images of Accell siRNA-GPER- or siRNA-control-transfected MCF-10A cells in a 3D culture through the middle acini, which were treated with E2 (32 nM, left panels) or control (0 nM, right panel) for 7 days. Laminin V (red); pan-cadherin (green). Scale bars = 20 mum. The presented blots were cropped. Full-length blots are presented in Supplementary Fig. 5 .