- MA5-15881 - Invitrogen Antibodies JUN antibody

Submitted references Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Cao YJ, Li HZ, Zhao J, Sun YM, Jin XW, Lv SQ, Luo JY, Fang XX, Wen WB, Liao JB
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2264394

Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.

Deng Y, Miki Y, Nakanishi A
Scientific reports 2020 Jan 28;10(1):1386

C6orf106 is a novel inhibitor of the interferon-regulatory factor 3-dependent innate antiviral response.

Ambrose RL, Liu YC, Adams TE, Bean AGD, Stewart CR
The Journal of biological chemistry 2018 Jul 6;293(27):10561-10573

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of c-Jun using a c-Jun monoclonal antibody (Product # MA5-15881) against a human c-Jun (AA: 199-331) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of c-Jun using a c-Jun monoclonal antibody (Product # MA5-15881) against a human c-Jun (AA: 199-331) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of c-Jun using c-Jun monoclonal antibody (Product # MA5-15881) in NIH/3T3 (1) and COS-7 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockout of c-JUN was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR905187_SGM ) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of c-JUN was performed by loading 30 µg of HEK-293 wild type (Lane1), HEK-293 Cas9 (Lane2), HEK-293 c-JUN KO (Lane3) modified whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with c-Jun Monoclonal Antibody (5B1) (Product # MA5-15881, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright™ FL1500 (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to c-JUN.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of PC-2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (Green). Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human Tunica intima cancer tissues using c-Jun monoclonal antibody (Product # MA5-15881) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun monoclonal antibody (Product # MA5-15881) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of HepG2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (blue) and negative control (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of HepG2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (blue) and negative control (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Analysis of E2 signal transduction. ( a ) cAMP assay showing cAMP levels (nM) in MCF-10A cells following treatment with 32 nM E2 for 15 min, 30 min, 24 h, and 48 h. Three independent experiments were performed. Bars represent +/-SD. ( b ) Western blotting of MCF-10A cells showing p38 and phospho-p38 (Thr180/Tyr182) following treatment with 32 nM E2 for 0-60 min. ( c ) Western blotting of MCF-10A cells treated with 32 nM E2 (left panel) or with 32 nM E2 and 20 nM G-15 (right panel) for 0-30 min. ( d ) Western blotting of MCF-10A cells showing JNK and phosphor-JNK (Thr183/Tyr185) following treatment with 32 nM E2 for 0-60 min. ( e ) Western blotting of MCF-10A cells treated with 32 nM E2 (left panel) or with 32 nM E2 and 20 nM G-15 (right panel) for 0-30 min. ( f ) Western blotting of MCF-10A cells treated with 32 nM E2 for 0-60 min showing IkB and phospho-IkB (Ser32, Ser36). ( g ) Western blotting of MCF-10A cells treated with 32 nM E2 for 0-60 min showing c-Jun and phospho-c-Jun (Ser63). ( h ) Representative confocal images of Accell siRNA-GPER- or siRNA-control-transfected MCF-10A cells in a 3D culture through the middle acini, which were treated with E2 (32 nM, left panels) or control (0 nM, right panel) for 7 days. Laminin V (red); pan-cadherin (green). Scale bars = 20 mum. The presented blots were cropped. Full-length blots are presented in Supplementary Fig. 5 .

MA5-15881

Antibody data