MAB6906

antibody from Novus Biologicals

Targeting: CREB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB6906

Provider

Novus Biologicals

Product name

Mouse Monoclonal CREB Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human CREB when phosphorylated at S133.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Phospho-CREB (S133) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm² ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-CREB (S133) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.