- 35-0900 - Invitrogen Antibodies CREB1 antibody

Submitted references mTOR Signaling Pathway Regulates the Release of Proinflammatory Molecule CCL5 Implicated in the Pathogenesis of Autism Spectrum Disorder.

Wang B, Qin Y, Wu Q, Li X, Xie D, Zhao Z, Duan S
Frontiers in immunology 2022;13:818518

Cannabinoid receptor 1 signaling in hepatocytes and stellate cells does not contribute to NAFLD.

Wang S, Zhu Q, Liang G, Franks T, Boucher M, Bence KK, Lu M, Castorena CM, Zhao S, Elmquist JK, Scherer PE, Horton JD
The Journal of clinical investigation 2021 Nov 15;131(22)

Central administration of afzelin extracted from Ribes fasciculatum improves cognitive and memory function in a mouse model of dementia.

Oh SY, Jang MJ, Choi YH, Hwang H, Rhim H, Lee B, Choi CW, Kim MS
Scientific reports 2021 Apr 28;11(1):9182

Menin and PRMT5 suppress GLP1 receptor transcript and PKA-mediated phosphorylation of FOXO1 and CREB.

Muhammad AB, Xing B, Liu C, Naji A, Ma X, Simmons RA, Hua X
American journal of physiology. Endocrinology and metabolism 2017 Aug 1;313(2):E148-E166

Acetyl CoA Carboxylase Inhibition Reduces Hepatic Steatosis but Elevates Plasma Triglycerides in Mice and Humans: A Bedside to Bench Investigation.

Kim CW, Addy C, Kusunoki J, Anderson NN, Deja S, Fu X, Burgess SC, Li C, Ruddy M, Chakravarthy M, Previs S, Milstein S, Fitzgerald K, Kelley DE, Horton JD
Cell metabolism 2017 Aug 1;26(2):394-406.e6

Long-term treatment with standardized extract of Ginkgo biloba L. enhances the conditioned suppression of licking in rats by the modulation of neuronal and glial cell function in the dorsal hippocampus and central amygdala.

Oliveira DR, Sanada PF, Filho AC, Conceição GM, Cerutti JM, Cerutti SM
Neuroscience 2013 Apr 3;235:70-86

Neuromodulatory property of standardized extract Ginkgo biloba L. (EGb 761) on memory: behavioral and molecular evidence.

Oliveira DR, Sanada PF, Saragossa Filho AC, Innocenti LR, Oler G, Cerutti JM, Cerutti SM
Brain research 2009 May 7;1269:68-89

Inactivation of CREB mediated gene transcription by HDAC8 bound protein phosphatase.

Gao J, Siddoway B, Huang Q, Xia H
Biochemical and biophysical research communications 2009 Jan 30;379(1):1-5

Schoenheimer effect explained--feedback regulation of cholesterol synthesis in mice mediated by Insig proteins.

Engelking LJ, Liang G, Hammer RE, Takaishi K, Kuriyama H, Evers BM, Li WP, Horton JD, Goldstein JL, Brown MS
The Journal of clinical investigation 2005 Sep;115(9):2489-98

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CREB was performed by loading 20 µg of HeLa (lane1), Hep G2 (lane2), NIH\3T3 (lane3), Mouse Testis (lane4), C2C12 (lane5) and U2OS (lane6) cell lysate using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. CREB was detected at ~43 kDa using CREB Mouse Monoclonal Antibody (Product # 35-0900) at 1-3 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CREB was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with CREB Mouse Monoclonal Antibody (Product # 35-0900) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Immunofluorescence analysis of brain sections from the hippocampus. Standard C57BL/6 mice (4 weeks old) were pre-treated into third-ventricle of brains cannula with 100 ng/ul of Afzelin (0.5 ul) or PBS (0.5 ul) for one month. The mice were received with scopolamine (Scop) injection (i.p. 0.8 mg/kg) for the behavior tests then hippocampi of these mice were collected for immunofluorescence analysis. The representative images of immunoreactivity to CREB expression in the hippocampal CA3 region were shown with CREB (red) and DAPI (blue) using confocal microscopes ( A ). These images were quantified to bar graph in the control (PBS as a vehicle), scopolamine + vehicle (Scop + vehicle, PBS as a vehicle) and scopolamine + Afzelin (Scop + Afzelin, 100 ng/ul afzelin of pretreatments) groups ( B ). Scale bars, 100 mum. The fluorescence intensity was quantified using Image J. *p < 0.05 **p < 0.01; one-way ANOVA with Dunnett's post hoc test; n = 3 for quantified bar graph ( B ). Data are mean +- s.e.m.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Cross-talk between mTOR signaling cascades, NF-kappaB, and CREB regulates the production of CCL5. HMC3 cells were treated with rapamycin (Rapa, 0-600nM) for 48 h, and the activation of NF-kappaB and mTOR signaling was assessed. (A) The total and phosphorylated levels of p65 subunit of NF-kappaB were analyzed by Western blotting, and GAPDH served as the loading control. (B) The total and phosphorylated levels of CREB were analyzed by Western blotting, and (C) active CREB (pCREB) protein level is further quantified (n=5 each group). (D) Transcriptional cross-talk between CREB, NF-kappaB, and mTOR pathway. NSC 228155 rescued the mTOR inhibitor (rapamycin)-mediated reduction of the gene expression level of CCL5, while p65-siRNA further counteracted NSC 228155's stimulating effect on CCL5 expression. The gene expression level of the CCL5 was determined by qRT-PCR (n=3 each group). (E) NSC 228155 reversed the repressive effect of rapamycin on CCL5 production. The concentration of CCL5 protein in the cell culture medium was measured by ELISA (n=3 each group). (F) The total and phosphorylated levels of GSK-3beta were analyzed by Western blotting. Data are presented as mean +- SD. Statistical significance was determined by Student- t test and denoted by * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

35-0900

Antibody data