Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36843 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-CREB (Ser129) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 38 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Scaffold Protein Axin Promotes Signaling Specificity within the Wnt Pathway by Suppressing Competing Kinase Reactions.
Gavagan M, Fagnan E, Speltz EB, Zalatan JG
Cell systems 2020 Jun 24;10(6):515-525.e5
Cell systems 2020 Jun 24;10(6):515-525.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-CREB (Ser129) in Lane 1: A375 whole cell lysate (40 µg), Lane 2: MCF-7 whole cell lysate (40 µg), Lane 3: the Brain tissue lysate of rat (40 µg), Lane 4: BV2 whole cell lysate (40 µg). Samples were incubated with Phospho-CREB (Ser129) polyclonal antibody (Product # PA5-36843) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-CREB pSer129 using Phospho-CREB pSer129 polyclonal antibody (Product # PA5-36843) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with UV (4h), Lane 2: Raw264.7 cell lysate treated with UV (24h), Lane 3: PC12 cell lysate treated with UV (4h).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-CREB pSer129 in paraffin-embedded human breast carcinoma using Phospho-CREB pSer129 polyclonal antibody (Product # PA5-36843) at a dilution of 1:100.