M00577

antibody from Boster Biological Technology

Targeting: CREB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00577

Provider

Boster Biological Technology

Product name

Anti-CREB CREB1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for CREB/CREB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat CREB/CREB1 information: Molecular Weight: 36688 MW; Subcellular Localization: Nucleus .

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DCO-3

Vial size

100ug/vial

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of CREB expression in NIH/3T3 cell lysate (M00577). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 monoclonal antibody (Catalog # M00577) overnight at 4

Additional image