Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM00027PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00027PU-N, RRID:AB_973050
- Product name
- anti CREB + ATF1 pSer133/Ser63 (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Synthetic phosphopeptide conjugated to KLH.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10E9
- Vial size
- 0.1 mg
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Phosphospecificity Whole cell extracts of control (1), EGF stimulated (2) or pervanadate treated (3) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab CREB/ATF1-10E9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
