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- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN951694 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-CAMP Responsive Element Binding Protein 1 (CREB1) (AA 112-140), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 112-140 amino acids from the Central region of human CREB1
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 112-140,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Common variants in 40 genes assessed for diabetes incidence and response to metformin and lifestyle intervention in the diabetes prevention program.
Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Jablonski KA, McAteer JB, de Bakker PI, Franks PW, Pollin TI, Hanson RL, Saxena R, Fowler S, Shuldiner AR, Knowler WC, Altshuler D, Florez JC, Diabetes Prevention Program Research Group
Diabetes 2010 Oct;59(10):2672-81
Diabetes 2010 Oct;59(10):2672-81
Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S, DREAM investigators
Diabetes care 2010 Oct;33(10):2250-3
Diabetes care 2010 Oct;33(10):2250-3
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of CREB1 (arrow) using rabbit polyclonal CREB1 Antibody (Center) (Cat. #AP51071PU-N). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CREB1 gene.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- CREB1 Antibody (Center) (Cat. #AP51071PU-N) western blot analysis in mouse cerebellum tissue lysates (35μg/lane).This demonstrates the CREB1 antibody detected the CREB1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CREB1 Antibody (Center)(Cat#AP51071PU-N) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- CREB1 Antibody (Center) (Cat. #AP51071PU-N) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
