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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB300-273 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-273, RRID:AB_10002306
- Product name
- Rabbit Polyclonal CREB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Effects of prolyl-hydroxylase inhibition and chronic intermittent hypoxia on synaptic transmission and plasticity in the rat CA1 and dentate gyrus.
Altered ERK/MAPK signaling in the hippocampus of the mrsk2_KO mouse model of Coffin-Lowry syndrome.
What turns CREB on?
CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events.
CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events.
CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals.
CREB and memory.
Transcriptional regulation by cyclic AMP.
Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus.
Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus.
Wall AM, Corcoran AE, O'Halloran KD, O'Connor JJ
Neurobiology of disease 2014 Feb;62:8-17
Neurobiology of disease 2014 Feb;62:8-17
Altered ERK/MAPK signaling in the hippocampus of the mrsk2_KO mouse model of Coffin-Lowry syndrome.
Schneider A, Mehmood T, Pannetier S, Hanauer A
Journal of neurochemistry 2011 Nov;119(3):447-59
Journal of neurochemistry 2011 Nov;119(3):447-59
What turns CREB on?
Johannessen M, Delghandi MP, Moens U
Cellular signalling 2004 Nov;16(11):1211-27
Cellular signalling 2004 Nov;16(11):1211-27
CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events.
Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia Z, Greenberg ME
Neuron 2002 Apr 11;34(2):221-33
Neuron 2002 Apr 11;34(2):221-33
CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events.
Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia Z, Greenberg ME
Neuron 2002 Apr 11;34(2):221-33
Neuron 2002 Apr 11;34(2):221-33
CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals.
Shaywitz AJ, Greenberg ME
Annual review of biochemistry 1999;68:821-61
Annual review of biochemistry 1999;68:821-61
CREB and memory.
Silva AJ, Kogan JH, Frankland PW, Kida S
Annual review of neuroscience 1998;21:127-48
Annual review of neuroscience 1998;21:127-48
Transcriptional regulation by cyclic AMP.
Montminy M
Annual review of biochemistry 1997;66:807-22
Annual review of biochemistry 1997;66:807-22
Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus.
Karin M, Hunter T
Current biology : CB 1995 Jul 1;5(7):747-57
Current biology : CB 1995 Jul 1;5(7):747-57
Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus.
Karin M, Hunter T
Current biology : CB 1995 Jul 1;5(7):747-57
Current biology : CB 1995 Jul 1;5(7):747-57
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CREB [p Ser133] Antibody [NB300-273] - Western blot of forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left lane). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase(lambda-Ptase, 1200 units for 30 min).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.
