PA5-36600

antibody from Invitrogen Antibodies

Targeting: MT-ND5 MTND5, NAD5, ND5

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36600

Provider

Invitrogen Antibodies

Product name

MT-ND5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 67 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cyclophilin D-dependent mitochondrial permeability transition amplifies inflammatory reprogramming in endotoxemia.

Veres B, Eros K, Antus C, Kalman N, Fonai F, Jakus PB, Boros E, Hegedus Z, Nagy I, Tretter L, Gallyas F Jr, Sumegi B
FEBS open bio 2021 Mar;11(3):684-704

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

7 Fig. CypD deficiency attenuates LPS-induced alterations regarding processes of mitochondrial replication in the sterile shock model. (A) Effect of CypD deficiency on the LPS-induced alterations of regulators of transcription from mtDNA. Green symbols represent downregulated genes, while white symbols show genes not significantly affected. (B) Fold change values of LPS-induced DEGs in the mitochondrial transcription regulators and mtDNA-encoded genes in WT and CypD -/- mice. Green bars represent downregulated genes gray bars show genes not significantly affected, ( n = 5), Kal's Z -test, adjPval < 0.05, [FC] > 1.5. (C) Bar graph demonstrating DEGs for mitochondrial ribosomes and accessory proteins in LPS-exposed WT and CypD -/- mouse liver tissues. Green bars represent downregulated genes gray bars show genes not significantly affected, ( n = 5), Kal's Z -test, adjPval < 0.05, [FC] > 1.5. (D) The effect of CypD disruption on LPS-induced changes of mtDNA levels relative to nuclear DNA content in liver tissues during endotoxemia, determined by qPCR. Data are presented as mean +- SEM, ( n = 5), * P < 0.05, one-way ANOVA followed by Bonferroni post hoc comparisons. (E) Western blot analysis of the effect of CypD disruption after LPS exposure on the cellular level of mitochondrial protein complex I component, mtND5. Data are presented as mean +- SEM ( n = 5), * P < 0.05, one-way ANOVA followed by Bonferroni post hoc comparisons.