Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- F54903 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NADH-ubiquinone oxidoreductase chain 5 Antibody / MT-ND5
- Antibody type
- Polyclonal
- Description
- This highly specific NADH-ubiquinone oxidoreductase chain 5 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the NADH-ubiquinone oxidoreductase chain 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) CCRF-CEM and 2) Jurkat cell lysate with NADH-ubiquinone oxidoreductase chain 5 antibody. Expected molecular weight: 65-70 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human CCRF-CEM cells with NADH-ubiquinone oxidoreductase chain 5 antibody; Blue=isotype control, Green= NADH-ubiquinone oxidoreductase chain 5 antibody.