Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-109993 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MT-ND6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: LIREDPIGAG ALYDYGR
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.34 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer.
Sideroflexin 4 is a complex I assembly factor that interacts with the MCIA complex and is required for the assembly of the ND2 module.
Zhu J, Zheng X, Lu D, Zheng Y, Liu J
Oxidative medicine and cellular longevity 2022;2022:8038857
Oxidative medicine and cellular longevity 2022;2022:8038857
Sideroflexin 4 is a complex I assembly factor that interacts with the MCIA complex and is required for the assembly of the ND2 module.
Jackson TD, Crameri JJ, Muellner-Wong L, Frazier AE, Palmer CS, Formosa LE, Hock DH, Fujihara KM, Stait T, Sharpe AJ, Thorburn DR, Ryan MT, Stroud DA, Stojanovski D
Proceedings of the National Academy of Sciences of the United States of America 2022 Mar 29;119(13):e2115566119
Proceedings of the National Academy of Sciences of the United States of America 2022 Mar 29;119(13):e2115566119
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4. SFXN4 mediates the incorporation of MT-ND6 into complex I. ( A ) Mitochondrial lysates from control and SFXN4 KO cells were analyzed by SDS-PAGE and immunoblotting. ( B ) Mitochondria isolated from control and SFXN4 KO + FLAG SFXN4 cells were solubilized in 1% digitonin and subjected to anti-FLAG IP. Eluates were analyzed by BN-PAGE and immunoblotting. ( C ) Mitochondria isolated from NDUFAF1 KO and NDUFAF1 KO + FLAG SFXN4 cells were solubilized in 1% digitonin and subjected to anti-FLAG IP. Eluates were analyzed by SDS-PAGE and immunoblotting. ( D ) Anti-FLAG IP was performed using mitochondria isolated from control (ACAD9 KO + ACAD9 FLAG ) and ACAD9 + ACAD9 FLAG SFXN4 KO cells. Eluates were analyzed by label-free quantitative MS. The volcano plot depicts the levels of ACAD9-interacting partners in SFXN4 KO cells relative to control cells ( n = 3). Proteins on the right of the volcano were more abundant in the ACAD9 FLAG IP from the SFXN4 KO cell line, and proteins on the left of the volcano were more abundant in the ACAD9 FLAG precipitation from the control cell line. Horizontal cutoff represents a P value of 0.05, and vertical cutoffs represent 1.5x fold change. Proteins are color coded according to the complex I assembly module to which they belong.
