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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 650002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#650002, RRID:AB_10708878
- Product name
- Purified anti-Cytochrome b-245 light chain
- Antibody type
- Monoclonal
- Antigen
- Partially purified human neutrophil flavocytochrome b
- Description
- The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. The p22phox gene encodes the light alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. p22phox is a prerequisite for TNF-induced but not for Toll-like-receptor-induced ROS production. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD) that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human neutrophil extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-p22phox antibody (clone 44.1). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
