BS1392

antibody from Bioworld Technology, Inc
Targeting: CCNB1 CCNB
Provider product page for BS1392
Western blot
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
BS1392 - Provider product page
Provider
Bioworld Technology, Inc
Proper citation
Bioworld Technology Cat#BS1392, RRID:AB_1663940
Product name
Cyclin B1 (I120) polyclonal antibody
Antibody type
Polyclonal
Antigen
Synthetic peptide, corresponding to amino acids 90-140 of Human Cyclin B1.
Description
In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13 SUC1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has estalished that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Isotype
IgG
Vial size
100ul
Concentration
1 mg/ml
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
CCNB1 protein structure - BS1392 shown in red.